Course in Gene Manipulation

Course

In Coimbatore

Price on request

Description

  • Type

    Course

  • Location

    Coimbatore

Objectives: To expose the students to current techniques in gene manipulation. Goal: After successful completion of this paper students will have thorough knowledge in. manipulation of DNA

Facilities

Location

Start date

Coimbatore (Tamil Nādu)
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Bharathiar University, Coimbatore, 641046.

Start date

On request

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Course programme

Completion of this paper will help the students to understand more about the application
of genetic engineering in plants, animals and medicine.
Objectives:
To expose the students to current techniques in gene manipulation
Goal:
After successful completion of this paper students will have thorough knowledge in
manipulation of DNA
Contents
Unit I:
Organization of genome - human, plant, animal and microbial genome. whole genome
assembly. Enzymes in Molecular Biology: Nucleases, Restriction endonucleases, DNA
Ligases, topoisomerases, gyrases, methylases, other modifying enzymes
Unit II:
Gene cloning vectors- Biology construction of Plasmids, lambda Bacteriophages,
Cosmids, Phasmids vectors. Binary and Co integrative vectors.
Unit III:
Basic Techniques in Gene Manipulation: Isolation and purification of Nucleic Acids -
Agarose Gel Electrophoresis; Blotting - Southern, Northern and Western blotting
Unit IV:
Gene cloning techniques: cloning strategies; Screening techniques of recombinants:
Direct methods and indirect methodsUnit V:
Characterization of transcriptome- micro arrays, DNA chips and SAGE technology. DNA
Profiling/DNA fingerprinting: DNA Markers in disease diagnosis and finger printing:
RFLPs, VNTRs, Microsatellites, SNPs, Current Technology for DNA Finger printing.

Course in Gene Manipulation

Price on request