Course in Gene Manipulation

Bharathiar University
In Coimbatore

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Important information

  • Course
  • Coimbatore
Description

Objectives: To expose the students to current techniques in gene manipulation. Goal: After successful completion of this paper students will have thorough knowledge in. manipulation of DNA

Important information
Venues

Where and when

Starts Location
On request
Coimbatore
Bharathiar University, Coimbatore, 641046., Tamil Nadu, India
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Course programme

Completion of this paper will help the students to understand more about the application
of genetic engineering in plants, animals and medicine.
Objectives:
To expose the students to current techniques in gene manipulation
Goal:
After successful completion of this paper students will have thorough knowledge in
manipulation of DNA
Contents
Unit I:
Organization of genome - human, plant, animal and microbial genome. whole genome
assembly. Enzymes in Molecular Biology: Nucleases, Restriction endonucleases, DNA
Ligases, topoisomerases, gyrases, methylases, other modifying enzymes
Unit II:
Gene cloning vectors- Biology construction of Plasmids, lambda Bacteriophages,
Cosmids, Phasmids vectors. Binary and Co integrative vectors.
Unit III:
Basic Techniques in Gene Manipulation: Isolation and purification of Nucleic Acids -
Agarose Gel Electrophoresis; Blotting - Southern, Northern and Western blotting
Unit IV:
Gene cloning techniques: cloning strategies; Screening techniques of recombinants:
Direct methods and indirect methodsUnit V:
Characterization of transcriptome- micro arrays, DNA chips and SAGE technology. DNA
Profiling/DNA fingerprinting: DNA Markers in disease diagnosis and finger printing:
RFLPs, VNTRs, Microsatellites, SNPs, Current Technology for DNA Finger printing.

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