Diploma in Biological Cloning

Bharathiar University
In Coimbatore

Price on request
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Important information

Typology Diploma
Start Coimbatore
  • Diploma
  • Coimbatore

Important information

Where and when

Starts Location
On request
Bharathiar University, Coimbatore, 641046., Tamil Nadu, India
See map
Starts On request
Bharathiar University, Coimbatore, 641046., Tamil Nadu, India
See map

Course programme

Plasmids (pUC 18 and Ti plasmids), Bacteriophages (l phage), Plasmids, Cosmids (pJB8),
SV40, retrovirus and Artificial Chromosomes (BAC, YAC).
Strategies in gene cloning: restriction, ligase, insertion into vector, cloning, transformation
into host cell, Enzymology of Recombinant DNA.
Screening for recombinant (Insertional inactivation, Colony/in situ hybridization,
radioactive antibody test, Xgal, complementation and physical methods)
07BIDT2 Diploma Paper - II: Methods of gene transfer and genome sequencing
CaPO4 mediated gene transfer, liposomes, electroporation, electro fusion, micro-injection,
particle bombardment.
DNA sequencing (Sanger and Coulson method; Maxam and Gilbert method and
Automated method) - Chromosomal walking, transposons, construction of genomic and
cDNA libraries; molecular markers- RAPD, RFLP.
07BIDT3 Diploma Paper -III : Applications of rDNA technology
Transgenic plants - high yielding, salt, draught, herbicide, disease resistant.
Transgenic animals - for improved livestock production.
rDNA in medicine: Vaccines, enzymes, blood factors, interferon, gene therapy,
DNA fingerprinting and its applications in forensic sciences.
Diploma Practical I
1. Pure culture techniques
2. Preparing competent culture
3. Agarose gel electrophoresis
5. Blotting techniques
6. PCR
07BIDP2 Diploma Practical II - rDNA Technology
1. Microbial genomic DNA isolation
2. Microbial plasmid isolation
3. Plant genomic DNA isolation
4. Animal genomic DNA isolation
5. Restriction mapping with Lamda DNA
6. Ligation

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