Diploma in Protemics
Diploma
In Coimbatore
Description
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Type
Diploma
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Location
Coimbatore
Goals: Proteomics is extensively used in drug discovery, and in learning various tools for analysis. of proteins. Objectives: Taxonomy, structure-function relationship and functional aspects of the entire set cell.
Facilities
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Start date
Start date
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Course programme
Course number : 07BIC08 Number of lecture hours: 5
Subject description :
This paper deals with protein structure prediction and function and various tools for
analysis of proteins.
Goals:
Proteomics is extensively used in drug discovery, and in learning various tools for analysis
of proteins.
Objectives:
Taxonomy, structure-function relationship and functional aspects of the entire set cell
UNIT - I
Protein classification: Structural elements and terminology, Helix, Sheet, Strand, Loop
and coil, Active site, Architecture, Blocks, Class and Domains, Fold, Motif, PSSM, Profile.
Principles of classification: Based on structural features, Phylogenetic relationship, CATH
- Classification by Class, Architecture, Topology, Homology, SCOP - Structural
Classification of Protein, FSSP - Fold classification based on structure -
structure alignment, MMDB - Molecular Modeling Database, SARF - Spatial
arrangement of backbone fragments
UNIT - II
Protein structure prediction: Use of sequence pattern, leucine zipper, coiled
coil, transmembrane, signal peptide, cleavage site. Secondary structure prediction:
Chou - Fasman / GOR method, Neural network, nearest neighbor method, tertiary
structure prediction, threading profile, contact potential, modeling.
UNIT - III
Analytical protein and peptide separations - Complex protein and peptide mixtures,
Extracting proteins from biological samples, Protein separation before digestion: 1D and 2
D Electrophoresis, Immobilized pH gradient, Sample preparation, First dimension criteria,
second dimension criteria, Stabilization, Detecting protein on gel: Electro blot, Image
analysis, Digital imaging, Spot detection and quantification, Gel matching. Data Analysis -
Database for 2D gel.
UNIT - IV
Tools of Proteomics-
Mass Spectrometry for protein and peptide analysis:
- MALDI-TOF Analyzers
- ESI Tandem MS instrument
- Tandem Mass Analyzers
- The Triple Quadrupole Mass Analyzer
- The Ion Trap Mass Analyzer
- Q-TOF & Fourier Transform-Ion Cyclotron Resonance MS Instrument
UNIT - V
Functional Proteome Analysis: Integrated Proteome Analysis - Phage antibody as tool,
Protein expression analysis, High throughput analysis for proteomics. Automation of
proteomic analysis. Proteomics in plant breeding: Objectives,principles and methods,
Genetic diversity analysis, Distribution of varieties, lines andcultivars, Mutant
characteristics, Variability between organ and developmental stage, Identification of abiotic
stress, Genetic mapping of protein markers.
Diploma in Protemics