M.Pharma Pharmaceutical Chemistry

Unit – I

Electromagnetic spectrum, UV-visible range, structural
features, absorption of radiant energy, factors influencing absorption of radiant
energy: Instrumentation — single-beam spectrophotometer, double-beam
spectrophotometer, Assay methods, Applications in pharmaceutical analysis.
Infrared Spectroscopy: Molecular vibrations, stretching vibrations, bending
vibrations, vibrational frequencies, factors influencing vibrational frequencies,
electronic effects, Instrumentation— Single-monochromator IRspectrophotometer,
experimental profile of IR-spectroscopy : Quantitative
analysis, applications in the analysis of Pharmaceutical dosage forms, qualitative
interpretation of IR-spectra, Recent advances in IR-spectroscopy, e.g. FT-IR,
ATR, etc. Optical Rotatory Dispersion: Fundamental principles of ORD, Cottoneffect
curves — their characteristics and interpretation, Octet rule and its
applications, circular dichroism.

Unit – II

Nuclear Magnetic Resonance Spectroscopy: The NMR-phenomenon viz. spinning
nucleus, effect of an external field, precessional motion, precessional frequency,
energy transition, chemical shift, 3H-NMR (Tritium NMR-spectroscopy), 13CNMR-
spectroscopy, 2D-NMR, interpretations of NMR-spectrum, instrumentation,
applications in pharmaceutical analysis. Mass Spectrometry: Basic principles and
brief outline of instrumentation, ion formation and types: molecular ion, metastable
ions, fragmentation processes. Fragmentation patterns and fragment
characteristics in relation to parent structure and functional groups; Mass
spectrum, its characteristics, presentation and interpretation. Recent advances in
MS, viz. GC-MS, chemical ionization MS and Fast Atom Bombardment Mass
Spectroscopy.