M.Sc. Biotechnology:Plant Biotechnology and Tissue Culture

Thapar University
In Patiala

Price on request
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Important information

  • Master
  • Patiala
  • Duration:
    2 Years

Important information

Where and when

Starts Location
On request
Thapar University P.O Box 32, 147004, Punjab, India
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Course programme

M.Sc. Biotechnology:Plant Biotechnology and Tissue Culture

First: Semester

General Biology (For non -medical group)
Basic Mathematics (For medical group)
Microbial Physiology and Genetics
Biostatistics and Bioinformatics
Cell and Molecular Biology

Second: Semester

Genetic and Metabolic Engg.
Bioprocess Technology
Immunology and Virology
Food Biotechnology
Agricultural & Industrial Microbiology

Summer Training – 6 weeks (During Summer vacations)

Third Semester

Summer Training (Presentation)
Entrepreneurship and Biosafety
Thesis (starts)

Fourth Semester


Plant Biotechnology and Tissue Culture

Introduction & scope of plant biotechnology; Architecture of plant nuclear, chloroplast and mitochondrial genome; Strategies of molecular cloning of plant genes; Agrobacterium mediated genetic engineering in plants (Ti and Ri-plasmid vectors); Plant viruses as vectors; Direct transformation of plants (electroporation, particle gun bombardment); Crop improvement and manipulation of phenotypic traits in plants using recombinant DNA approach (herbicide resistance, virus resistance, pest resistance, resistance towards fungal pathogens & stress tolerance); Quality and quantity improvement of plant oils, starch and storage protein; Genes responding to hormones, phytochrome, abiotic and biotic stresses; Chloroplast transformation; Basic genetics and principles of plant breeding; Molecular marker-aided breeding;

Plant metabolic engineering and industrial products: Plant secondary metabolites, control mechanisms and manipulation of phenylpropanoid pathways, shikimate pathway, alkaloids, industrial enzymes, polyhydroxy butyrate, therapeutic proteins, antibodies, edible vaccines, purification strategies, oliosin partitioning technology; The ethical and social impact of plant biotechnology.

Plant cell, tissue and organ culture: History, rapid clonal propagation techniques, media components, organogenesis and somatic embryogenesis; anther culture; protoplast isolation and culture; Bioresource conservation.

Laboratory: Plant tissue culture media, explant preparation, callus induction, regeneration and morphogenesis, meristem culture for virus free plants, protoplast isolation, micropropagation and green house hardening; Isolation and purification of plant DNA and RNA, restriction analysis of plant DNA, transfer of Ti plasmid into Agrobacterium, Agrobacterium-mediated transformation of plants, electroporation techniques

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