M.Sc. Biotechnology:Plant Biotechnology and Tissue CultureThapar University
Price on request
M.Sc. Biotechnology:Plant Biotechnology and Tissue Culture
General Biology (For non -medical group)
Basic Mathematics (For medical group)
Microbial Physiology and Genetics
Biostatistics and Bioinformatics
Cell and Molecular Biology
Genetic and Metabolic Engg.
Immunology and Virology
Agricultural & Industrial Microbiology
Summer Training – 6 weeks (During Summer vacations)
Summer Training (Presentation)
Entrepreneurship and Biosafety
Plant Biotechnology and Tissue Culture
Introduction & scope of plant biotechnology; Architecture of plant nuclear, chloroplast and mitochondrial genome; Strategies of molecular cloning of plant genes; Agrobacterium mediated genetic engineering in plants (Ti and Ri-plasmid vectors); Plant viruses as vectors; Direct transformation of plants (electroporation, particle gun bombardment); Crop improvement and manipulation of phenotypic traits in plants using recombinant DNA approach (herbicide resistance, virus resistance, pest resistance, resistance towards fungal pathogens & stress tolerance); Quality and quantity improvement of plant oils, starch and storage protein; Genes responding to hormones, phytochrome, abiotic and biotic stresses; Chloroplast transformation; Basic genetics and principles of plant breeding; Molecular marker-aided breeding;
Plant metabolic engineering and industrial products: Plant secondary metabolites, control mechanisms and manipulation of phenylpropanoid pathways, shikimate pathway, alkaloids, industrial enzymes, polyhydroxy butyrate, therapeutic proteins, antibodies, edible vaccines, purification strategies, oliosin partitioning technology; The ethical and social impact of plant biotechnology.
Plant cell, tissue and organ culture: History, rapid clonal propagation techniques, media components, organogenesis and somatic embryogenesis; anther culture; protoplast isolation and culture; Bioresource conservation.
Laboratory: Plant tissue culture media, explant preparation, callus induction, regeneration and morphogenesis, meristem culture for virus free plants, protoplast isolation, micropropagation and green house hardening; Isolation and purification of plant DNA and RNA, restriction analysis of plant DNA, transfer of Ti plasmid into Agrobacterium, Agrobacterium-mediated transformation of plants, electroporation techniques