Master Of Medicine (Biochemistry)

Suamandeep Vidyapeeth University
In Vadodara

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Important information

  • Master
  • Vadodara
Description

Important information
Venues

Where and when

Starts Location
On request
Vadodara
At & Po Pipariya, Ta. Waghodia, Dist. Vadodara (Gujarat) India , 391760, Gujarat, India
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Course programme

Syllabus :

THEORY paper – I

PHYSICAL, ORGANIC ASPECTS OF BIOCHEMISTRY AND BIOCHEMICAL TECHNIQUES.



1. Electrolytes,pH buffer system, colloids, law of mass action, surface tension,osmosis. bioenergetics,diffusion, and molecular weight determnation.
2. Analytical Biochemistry 7 instrumentation, principles & application of colorimetry,fluorimetry, spectrophotometry, radio isotopic techniques, atomic absorption, spectroscopy, osmometry, centrifugation, nephelometry and chemiluminescence.
3. Bioseparative techniques.
Chromatography, column , paper, TLC, GLC, HPLC, affinity.
Electrophoresis- Paper, agarose, SDS, PAGE.
4. Protein conformation, interactions, structure activity relationship.
5. Only the description of accepted structures is required. Structure , physical & chemical properties of the following is required.

PRACTICALS

1. Estimation of proteins by Folin’ s method and dye binding method.
2. Titration of amino acid – formol titration & pK valus.
3. Two-dimensional Paper chromatography for separation of amino acids.
4. Ion exchange chromatography.
5. Paper electrophoresis.
6. Preparation & estimation of glycogen, cholesterol, casein and hemoglobin from biological samples.
7. Estimation of blood glucose and glucose tolerance test.
8. Estimation of Cholesterol, triglycerides, free fatty acids and phospholipids.
9. Estimation of electrolytes , pH and blood gas analysis.
10. Estimation of urea, Creatinine, Uric acid , ammonia including clearance tests.


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