Post Graduate in Bioinstrumentation and Biostatics

Bharathiar University
In Coimbatore

Price on request
You can also call the Study Centre
42224... More
Compare this course with other similar courses
See all

Important information

  • Post Graduate Diploma
  • Coimbatore
Description

Objective: To expose students to various bio physical technique and bio statistical tools. used in modern biotechnology research. Goal: Upon successful completion of the paper the students will get an insight on the. usage of various instruments and their relevant applications and presentation of their. results in statistical forms.

Important information
Venues

Where and when

Starts Location
On request
Coimbatore
Bharathiar University, Coimbatore, 641046., Tamil Nadu, India
See map

Course programme

This paper highlights on vital instrumentation techniques for measurement of
physical, physiological and biological factors in human and other living organisms and
also provides various statistical tools for the storage, retrieval and analysis of biological
data.
Objective: To expose students to various bio physical technique and bio statistical tools
used in modern biotechnology research.
Goal: Upon successful completion of the paper the students will get an insight on the
usage of various instruments and their relevant applications and presentation of their
results in statistical forms.
UNIT I
Microscopy: Light microscope, Fluorescence microscope, Phase contrast
microscope,Electron microscope, Confocal microscopy.
Centrifugation: Small bench top centrifuges, large capacity refrigerated centrifuges, High
speed refrigerated centrifuges, preparative and analytical ultra centrifuge.
Electrochemical techniques: Principles of electrochemical techniques, redox reactions,
the pH electrode, ion-sensitive and gas-sensitive electrodes, The clark oxygen electrode,
Biosensors. Spectrophotometry: visible and UV spectrophotometry.
UNIT II
Chromatographic teachniques: Principles of chromatography, size exclusion,
Ionexchange and affinigy chromatographies. High performance liquid chromatography
(HPLC), Gas liquid chromatography (GLC), Thin layer chromatography (TLC), Paper
chromatography, GC-MS, LC-MS, Maldi Tof.
Electrophoresis: General principles, Electrophoresis of proteins: SDS-PAGE , Native
gels, Gradient gel, Isoelectric focusing , 2-D gel electrphoresis (2-D PAGE), cellulose
acetate elecrophoresis, continuous flow electrophoresis; Detection, estimation and
recovery of proteins, Western blotting. Electrophoresis of nucleic acids: agarose gel
electrophoresis of DNA, DNA sequencing gels, Pulse field gel electrophoresis,
electrophoresis of RNA, Capillary electrophoresis.
UNIT III
Spectroscopic techniques: Properties of electromagnetic radiation, interaction with
matter. Gamma ray spectroscopy, X-ray spectroscopy, UV and Visible spectroscopy,
Infrared and Raman spectroscopy, Electron spin resonance spectroscopy, Nuclear
magnetic resonance spectroscopy, Circular dichorism spectroscopy,Atomic spectroscopy.
Lasers, Spectrofluorimetry, turbidometry and nephelometry.
UNIT IV
Radio isotope techniques: The nature of radioactivity, detection and measurement of
radioactivity: detection based on gas ionization- Geiger Muller counter- principles and
applications. Detection based on excitation- Liquid Scintillation counter-principle and
applications. Supply, storage and purity of radiolabelled compounds, specific activity,
inherent advantages and restrictions of radiotracer experiments, safety aspects,
applications of radio isotopes in biological sciences. Flowcytometry, ELISA,
immunoblotting.
UNIT V
Research methodology: Introduction to methodology, format of thesis and dissertation,
research articles, reviews, monographs, bibliography, literature search, citation literature.
Significance of research, research methods versus methodology, research and scientific
methods, defining research problem, research design.

Compare this course with other similar courses
See all