Post Graduate Diploma in Applied Health Sciences

Bharathiar University
In Coimbatore

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Important information

  • Post Graduate Diploma
  • Coimbatore
  • Duration:
    1 Year
Description

Important information
Venues

Where and when

Starts Location
On request
Coimbatore
Bharathiar University, Coimbatore, 641046., Tamil Nadu, India
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Course programme

PAPER: I - FOOD, NUTRITION AND PHYSIOLOGY
UNIT - I
Introduction - role of nutrients in human body - Nutritive requirements- General - Vitamins
and Minerals- Proteins, Carbohydrates, Lipids. Water and body fluids- energy balance.
UNIT - II
Organisation of alimentary canal- role of salivary glands, liver, pancreas and intestinal glands
in digestion- absorption.
Introduction to intermediate metabolism - carbohydrates - fat and protein - their types -
digestion, absorption and excretion.
UNIT - III
Nutritional deficiencies - diseases and their control - protein energy malnutrition, vitamin A
deficiency and nutritional blindness, anaemia, goiter and other nutritional diseases.
UNIT IV
Clinical Nutrition: Foods and their nutritive content- habitual diets in India and their
adequacy. Recommended dietary allowances - weight control - diets in diabetes mellitus-
Cardio vascular diseases - renal disease- GIT disease - cancer - stress.
UNIT V
Complimentary and alternative nutrition- Functional foods- phytochemicals and benefits
berries - chitin peppers- cruciferous vegetables, flax and fibres garlic family- herbs and
spices- legumes whole grains, nuts- orange and yellow fruits and vegetables, Tea, Red
grapes, tea and coffee- composition and benefits.
PRACTICALS:
1. Estimation of glucose
2. Estimation of Proteins (Biuret)
3. Estimation of Cholesterol
4. Estimation of Vitamin C (Ascorbic Acid)
5. Qualitative test for Calcium & Phosphorus
6. Qualitative test for Urea.
7. Recording of recommended dietary allowances for Indians.
8. Models: Stomach and Liver
PAPER: II - BIOCHEMISTRY
UNIT I
Amino Acids: Structure, Classification of amino acids and properties
Proteins: Classification of Proteins based on the chemical structure, properties
UNIT II
Carbohydrates: Structure, classification and properties of functional groups.
Lipids: Classification, properties - Saturated and unsaturated fatty acids - Cholesterol.
UNIT III
Enzymes: Classification, properties of enzymes, Mode of enzyme action, enzyme substrate
compounds.
UNIT IV
Nucleic acids: DNA structure and properties, DNA synthesis- Mechanism of replicationnucleotides
- Different types of RNA - mRNA and rRNA, tRNA.
UNIT V
Biomedical Instrumentation: Electrophoresis - Principle, Instrumentation, Applications ; UVSpectrophotometer-
Principal mode of action, Application; Centrifuge - Ultracentrifuge; PCR
Technique; Chromatography - Principle - Different types- Applications.
PRACTICALS:
Spectrophotometer /Calorimetric estimation of the following biomolecules
A. Total free amino acids (Ninhydrin reagent method)
B. Protein (Lowry et. al., 1951 Method
C. Total soluble carbohydrates (Anthrone reagent method)
D. RNA (Orcinal method)
E. DNA (Diphenylamine reagent method)
F. Paper Chromatographic separation of amino acids
G. Polyacrylamide gel electrophoretic separation of proteins - Demonstration.
H. Estimation of SGPT and SGOT in human blood
07ZOODA03 PAPER: III - OUTLINES OF IMMUNOLOGY
UNIT - I
Fundamental concept in immunology - Cells and organs of the immune system- Basics
Immunity- Types of Immunity - Structure and Types of immune system.
UNIT - II
Antigen - Antibody reaction - Immunoglobulins- Structure of Immunoglobulins - Biological
properties of Immunoglobulins classes- Immune response - Types of Immune response.
UNIT - III
Major Histo Compatibility complex (MHC)- Mechanism of Immune response - Salient features
and biosynthesis of complement- Hypersensitivity - Types of Hypersensitivity.
UNIT - IV
Cell mediated immune reaction - Regulation of immune response- Basics of tumour
immunology - Autoimmune disease.
UNIT - V
Classification of immunodeficiency disease. Immunological Techniques - Antigen - Antibody
interaction, Radioimmunoassay and Enzyme Linked ImmunoSorbent Assay.
PRACTICALS:
1. ELISA - Demonstration.
2. The widal test.
3. Blood group determination by slide agglutination; Rh factor determination by
agglutination reaction.
4. Differential count of blood; Total count of WBC and RBC.
5. Model: Antigen - Antibody Interaction.
07ZOODA04 PAPER: IV - HORMONES AND HEALTH
Health is important for everybody. Hormones are the important regulators of health and excess
or deficiency of them lead to many disorders. An understanding of their functioning helps in
alleviating and maintaining the health.
UNIT -I
Endocrine glands - basic classification of hormones - hormonal regulations, brief description of
location, structure and functions of pituitary, thyroid, parathyroid, pancreas, adrenal, Gonads
and pineal glands.
UNIT -II
Hypo and hyper functioning of glands e.g. Gigantism and Dwarfism, Diabetes Insipidus,
Cretinism, Goitre, Thyroiditis, Cushing's syndrome and Pheochromocytoma.
UNIT -III
Mineral and bone metabolism and role of vitamin D3, Calcitonin and Parathormone. Glucose
metabolism and the role of pancreatic islets - alpha and beta cells - diabetes mellitus,
hypertension. Hormones in food digestion and assimilation.
UNIT -IV
Hormones in reproduction - Puberty - Pregnancy - Hypogonadism, Infertility, Gonadal tumors,
Contraception in males and females. Cancer of the reproductive tract - Breast cancer and
Prostatic cancer.
UNIT -V
Hormones in behavior - Neuroendocrine - Motivation, Memory, Perception, Attention -
Schizophrenia, Mental depression.
PRACTICALS:
1. Spotters: Endocrine glands - pituitary, thyroid, adrenal, ovary, testes and thymus.
2. OGTT for glucose determination.
3. ELISA for hormone assay (Demonstration).
07ZOODA05 PAPER: V - ESSENTIALS OF CLINICAL CYTOGENETICS
UNIT-I
History of Human Chromosome Research - Denver Conference (1940) - Chicago Conference
(1966) - Paris Conference (1971) - Nomenclature of Human Chromosome.
UNIT-II
Identification of Human diploid chromosome - peripheral blood cultures - banding techniques -
G-band; Q-band; C-band; R-band - Identification of 23 pairs of Human chromosomes by band
position.
UNIT-III
Chromosomal syndromes: Autosomal syndromes - Sex chromosomal syndromes - Structural
chromosomal syndromes. Genetics of inborn errors of metabolism. Carbohydrate metabolism.
Galactosemia; Lipid metabolism; Phenylketonuria; Prenatal and postnatal diagnosis.
UNIT-IV
a) Prenatal diagnosis: Chorionic villi sampling - Foetoscopy, Ultrascopy - Amniocentesis. b)
Postnatal diagnosis: Peripheral blood leucocyte culture - Sister Chromatid Exchange - Fragile
site - Mitotic index. c) Genetic Counseling.
UNIT-V
Hereditary forms of Cancer - Oncogenes and Cancer - Chromosomes and Cancer - Cancer and the
environment.
PRACTICALS
1. Buccal smear test
2. Peripheral blood lymphocyte culture
3. Screening of Human chromosome
4. Micronucleus test.
07ZOODA06 Paper: VI -ENVIRONMENTAL HEALTH MICROBIOLOGY
UNIT - I: Viral Zoonoses
Zoonoses caused by alpha viruses and flavi viruses- Chikungunya fever _ Japanese Encephalitis
- Yellow fever- Dengue fever - Occurrence- Transmission - Clinical manifestations -
Diagnosis- Therapy.
UNIT - II: Bacterial Zoonoses
Leptospiroses- Plague- Rat bite fever- Para tuberculosis- Streptococcal infections- Cholera-
Occurrence - transmission- Clinical manifestations- Diagnosis- Therapy.
UNIT - III: Fungal Zoonoses
Introduction - Dermatophytoses caused by microsporum spp. - Dermatophytoses caused by
Trichophyton spp. - Sporotrichosis.
UNIT - IV: Parasitic Zoonoses
Zoonoses caused by Protozoa.
Amoebiasis - Giardiasis - Sleeping sickness - Filariasis - Trichinosis.
UNIT - V: Zoonoses caused by Arthropods
Diptera - Insect Bites caused by mosquitoes- Sand flies- Heteroptera bugs- Seimex species.
PRACTICALS:
1. Prepared slides of different microbes
2. Protozoan identification, observation (Serial dilution)
3. Simple staining, Gram staining
4. Slide culture techniques for studying fungus and molds
5. Preparation of bacterial smear and staining methods
6. Identification of different mosquito vector at Endemic zone
7. Human- host parasite interaction.
07ZOODA07 PAPER: VII - TOXICOLOGY AND HEALTH HAZARDS
UNIT - I
Origin - Scope - importance of Toxicology Toxicity - Acute & Chronic toxicity - Methods in
Toxicology- Bioassay
UNIT - II
Classification of Toxicants - Pesticides, heavy metals - Drugs of Cosmetics, Oil and combustion,
chemicals, Radioactive substances
UNIT - III
Route of exposure - Absorption - Distribution - Excretion - Metabolism of Toxicants
UNIT - IV
Mode of action of Xenobiotics - Target site and interactions - Factors affecting xenobiotics
chemicals. Persistence of Toxicants
Toxic residues - Residue analysis (Procedure & Techniques)
UNIT - V
Health hazards due to pollutants- air, water, land sources.
PRACTICALS:
1. Acute toxicity - Demonstration
(a) Surfacing activities (b) Convulsions
2. Determination of LC 50 using Finney's Method (1978)
3. Probit Analysis - Theory
4. LD 50 and EC50 - Theory
5. Effect of toxicants on tissues - Histopathology of Liver, Kidney and Muscle sections
6. Effect of toxicants on Hematological parameters - Haemoglobin content, WBC and
RBC counts
7. Effect of toxicants on enzymes, Acid and Alkaline Phosphatase
8. Antagonistic and synergistic effects - Theory
9. Effect of temperature on toxicity - Theory
10. Effect of pH on toxicity - Theory
Submission of slides:
Submission of 10 slides showing histopathology of tissues
07ZOODA08 PAPER: VIII - BIOMEDICAL INSTRUMENTATION
UNIT I:
Principles and application of Image analyser, UV visible spectrophotometer, Spectronic 20D+,
Flame Photometer, Semi-auto analyser and ELISA.
UNIT II:
Structure, Principles and functions of Glucometer, Sphygmomanometer, Centrifuges - tabletop
and ultracentrifuge, pH meter, Conductivity meter,
UNIT III:
Separation and Analytical Techniques: A) Chromatography: paper, thin-layer, column and
GLC. B) Electrophoresis: paper, disc gel and Immunoelectrophoresis
UNIT IV:
Sterilization techniques: Dry heat sterilization (Oven), Steam Sterilization (Autoclave),
Gaseous Sterilization, Radiation Sterilization (Ultraviolet Lamp).
UNIT V:
Computer Science: Classification - hardware, input and output devices, main and auxiliary
memories, CPU; Software application - MS Word.
PRACTICALS:
1. Operation of Image analyzer, UV/Vis Spectrophotometer, Spectronic 20D+, Flame
Photometer, Semi-auto analyzer, ELISA and pH meter.
2. Spotters: Glucometer, Sphygmomanometer, Electrophoresis unit, Chromatography unit,
Centrifuges, Oven and Autoclave.

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