POST GRADUATE DIPLOMA IN MEDICAL LABORATORY TECHNOLOGYMAHARASHTRA UNIVERSITY OF HEALTH SCIENCES
Price on request
- Post Graduate Diploma
POST GRADUATE DIPLOMA IN MEDICAL LABORATORY TECHNOLOGY
Clinical Biochemistry : Theory Portion :
(a) Introduction : Role of technician in the laboratory. Importance of Clinical Biochemistry
in medical field.
(b) Basic concepts of Biochemistry :
(These concepts should be dealt in brief, focussing on clinical relevance wherever
1. Carbohydrate.-.. Definition, classification, functions,properties, Osazone formation.
2. Proteins and amino acids: Definition, classification, functions, chemical reactions of
proteins & amino acids.
3. Nucleic acids : Definition, types, functions.
4. Lipids : Definition, classification, functions of lipids and lipoproteins.
5. Enzymes : Definition, classification, factors affecting enzyme action, inhibition,
diagnostic importance of enzyme and isoenzymes.
6. Carbohydrate metabolism : Definition and importance of Glycolysis, Glycogenesis,
Glyco-genolysis, Gluconeogenesis, Kreb's cycle, Cori's Cycle. Blood glucose regulation,
Glycosuria. Glucose tolerance tests
7. Protein metabolisin : Deamination and Transami-nation. Urea formation, Creatine
8. Lipid metabolism : Definition and importance of Beta oxidation of fatty acids, functions
and clinical importance of Triacylglycerol, Phospholipids and Cholesterol-Lipid profile
& its importance
9. Mineral metabolism : Functions and metabolism of Calcium, Phosphorous. Sodium.
Potassium. Iodine and Iron.
10. Blood : Composition, functions and separation of plasma proteins, Blood coagulation.
Chemistry and functions of haemoglobin including porphyrin and bilirubin metabolism.
11. Acid base balance : Definition and importance of blood pH and its maintenance by
buffers, Respiratory and renal mechanism, Acidosis and alkalosis (in brief).
12. Metabolis disorders : Diabetes Mellitus, Atherosclerosis,
13. Analytical instruments & techniques
Principle, types, uses , care & maintainance of
a) Photoelectric colorimeter, spectrophotometer ,flame photometer
14. Automation in clinical Biochemistry
Principle, types, uses of Autoanalyser ,Blood Gas analyzer & role of computer in
(c) Practical Aspects for Clinical Biochemistry:
1. Laboratory management and planning: Reception and recording of specimens,
maintenance of laboratory records, reporting.
2. Specimen collection : Whole blood, plasma, serum, urine, C.S.F & other body fluids,
preservation of specimens, anticoagulants.
3. Quality Control: Role of quality control and its importance. Accuracy, Reliability,
Precision. Internal and external quality control measure, preparation of reagents,
standardization of methods, safety measures and precautions.
4. Basic laboratory equipment's : Types, use, care and maintenance of following
equipment : Ovens, Incubators, Refrigerators, Deep freezer. Centrifuges, Waterbaths,
Water distillation ,Analytical Balances, microscopes.
5. Glasswares : Types, use, care and maintenance of flasks, pipettes, cylinders, funnels,
6. Analytical instruments and techniques : Principles, types, use, care and maintenance of
photoelectric colorimeters, spectrophotometers, flamephotometers, electrophoresis,
Chromatography, Elisa and RIA, isotopes.
7. Biochemical test profiles : Principle and use of Glucose tolerance test, liver function
tests, kidney function tests, Thyroid Function Test.
P ractical Course: -
Group A : Preparation, Standardization and estimation of following constituents in blood :
Glucose, urea, uric acid, creatinine, proteins (total proteins, A/G ratio), S.G.P.T., S.G.O.T.,
Group B : Estimation of following constituents in blood : (i) Acid phosphatase, Amylase,
bilirubin, calcium phosphates, Na, K, Cholesterol, Triacyl glycerol, prothrombin time. (ii)
C.S.F. (estimation of glucose, proteins, chlorides). (iii)Urine analysis.
Group C: Spots: Colour and precipitation reactions of proteins, instruments and appliances,
reagents, tests, electrophoresis, chromatography separation, G.T.T. curves.
V. Laboratory Training and Rotation :
Each candidates shall undergo training in laboratories, of all the three subjects.
Minimum duration of training in each subject shall be of clear 90 days (i.e. excluding
This period shall be utilized to help the candidates in learning practical and behavioral skills.
All the candidates will be divided into three batches. Then each batch will undergo training
in the three laboratories by rotation. Each candidate has to keep a record of his/her work
during training period. This period of training shall be utilized for helping the candidates to
learn the following :
1. Preparation and preservation of various reagents, stock standard solutions, buffer
solutions, anticoagulants for agulant solutions and bulbs for sample collection.
2. Use, care and maintenance of basic equipments, instruments and glass
3. Collection of samples of patients, receiving and registering the samples
4. Processing, recording and reporting of results.
5. Interpersonal relations and communication with doctors, nurses,
wardboys, patients, colleagues and superiors.