Advanced Proficiency courses in Plant Biotechnology(PART TIME)

Advanced Proficiency courses in Plant Biotechnology

Plant tissue culture is the in vitro technique of culturing a plant part viz. a protoplast, a cell, a tissue or an organ, in appropriate nutrient medium (static or liquid) aseptically. The science of plant tissue culture not only fulfills ones curiosity to understand plasticity and totipotency nature of plant cells, but being the most important part of plant biotechnology acts as a tool to produce millions of clonal plants in a small space within a short period of time and offers many diverse ways for plant improvement such as somaclonal variation, Agrobacterium mediated plant transformation etc.

Course Strength

The course aims at providing thorough practical exposure into different areas of plant Biotechnology and provides an excellent opportunity to work with agronomically, medicinally, and ornamentally important plants.

Course structure

Practicals
• Stock solution and Chemical preparation
• Media preparation
• In vitro culturing technique
• Primary Culture: - Seed, Embryo, Nodal, Internodal, Leaf , Storage organ, Nucellus, Anther, Shoot tip, Root tip, Suspension culture
• Sub culture: - Callus, Shoot induction, Root induction, Somatic embryo induction
• Somatic embryo viability test
• Production of Synthetic seeds
• Stress tolerance studies and biochemical estimations ( Salinity stress, Heavy metal stress, Drought stress)
• Protoplast isolation
• Agrobacterium mediated transformation
• Genomic DNA isolation from plant samples
• Plasmid DNA isolation from Agrobacterium
• Screening of transgenic plants using PCR
• RAPD analysis to analyze the chemically induced Somaclonal variation
• Secondary metabolite productiion, purification and confirmation through biochemical tests
• Histobiochemical GUS assay

Career Opportunities

As a research personnel in R& D departments
Provide consultancy to plant tissue culture industries
As entrepreneurs