Course in Protein Engineering

Unit I
Introduction to protein engineering conformation of proteins; structure prediction -
protein anatomy, secondary and super secondary structure; sequence, structure
relationship.
Unit II
Recombinant proteins using fusion protein strategies for enhanced recovery; engineering
protein for the affinity purification (engineering of streptavidin).
Unit III
Stabilization of enzymes by protein engineering (eg.Pseudomonas isoamylase,
Agrobacterium radiobacter); mutational effect on substrate specificity (Yeast
carboxypeptidase Y, glucoamylase).
Unit IV
Protein engineering in vaccine development. minibody; HCV protease; expression and
tagged cell surface receptors for the drug discovery.
Unit V
Engineering proteins for the degradation of recalcitrant compounds; environmentally
hazardous compounds.