M.Sc. Biotechnology:Plant Biotechnology and Tissue Culture

Thapar University
In Patiala

Price on request
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Important information

Typology Master
Location Patiala
Duration 2 Years
  • Master
  • Patiala
  • Duration:
    2 Years


Where and when

Starts Location
On request
Thapar University P.O Box 32, 147004, Punjab, India
See map
Starts On request
Thapar University P.O Box 32, 147004, Punjab, India
See map

Course programme

M.Sc. Biotechnology:Plant Biotechnology and Tissue Culture

First: Semester

General Biology (For non -medical group)
Basic Mathematics (For medical group)
Microbial Physiology and Genetics
Biostatistics and Bioinformatics
Cell and Molecular Biology

Second: Semester

Genetic and Metabolic Engg.
Bioprocess Technology
Immunology and Virology
Food Biotechnology
Agricultural & Industrial Microbiology

Summer Training – 6 weeks (During Summer vacations)

Third Semester

Summer Training (Presentation)
Entrepreneurship and Biosafety
Thesis (starts)

Fourth Semester


Plant Biotechnology and Tissue Culture

Introduction & scope of plant biotechnology; Architecture of plant nuclear, chloroplast and mitochondrial genome; Strategies of molecular cloning of plant genes; Agrobacterium mediated genetic engineering in plants (Ti and Ri-plasmid vectors); Plant viruses as vectors; Direct transformation of plants (electroporation, particle gun bombardment); Crop improvement and manipulation of phenotypic traits in plants using recombinant DNA approach (herbicide resistance, virus resistance, pest resistance, resistance towards fungal pathogens & stress tolerance); Quality and quantity improvement of plant oils, starch and storage protein; Genes responding to hormones, phytochrome, abiotic and biotic stresses; Chloroplast transformation; Basic genetics and principles of plant breeding; Molecular marker-aided breeding;

Plant metabolic engineering and industrial products: Plant secondary metabolites, control mechanisms and manipulation of phenylpropanoid pathways, shikimate pathway, alkaloids, industrial enzymes, polyhydroxy butyrate, therapeutic proteins, antibodies, edible vaccines, purification strategies, oliosin partitioning technology; The ethical and social impact of plant biotechnology.

Plant cell, tissue and organ culture: History, rapid clonal propagation techniques, media components, organogenesis and somatic embryogenesis; anther culture; protoplast isolation and culture; Bioresource conservation.

Laboratory: Plant tissue culture media, explant preparation, callus induction, regeneration and morphogenesis, meristem culture for virus free plants, protoplast isolation, micropropagation and green house hardening; Isolation and purification of plant DNA and RNA, restriction analysis of plant DNA, transfer of Ti plasmid into Agrobacterium, Agrobacterium-mediated transformation of plants, electroporation techniques

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