M.Tech. in Molecular Biology and Human Genetics

CELLULAR AND MOLECULAR BIOLOGY
Unit - I
Structure and organization of cell - Bacteria and Virus - Differences between
Prokaryotic and Eukaryotic cells, Structure, Composition and Function of Plasma
membrane, Mitochondria, Endoplasmic reticulum, Golgi complex, Vacuoles, Ribosomes,
Mircosomes, Cytoskeleton and Peroxisomes, Chemical composition and function of
Nucleus, Nucleolus and Chromosomes.
Unit - II
Overview of Cell cycle - mitosis - meiosis - Factors modifying the cell cycle - genes
regulating the cell cycle - microtubules - motile functions of microtubules -
microfilaments - microfilament based cell motility - nucleus and its molecular
constituents - organization of chromatin and cell nucleus
Unit III
Membrane Biogenesis - Lipid biogenesis and membrane asymmetry - membrane models
- active transport - passive transport - cell signaling - cell adhesion - cell junctions -
cellular oxidations and mitochondria - photosynthesis and chloroplast - chloroplast
transport
Unit IV
Development of motile male gametes - development of female gametes - fertilization in
animals - gametogenesis and fertilization in higher plants
Unit V
Characteristics of Cancer - establishing the genetic basis of cancer - viruses and cancer -
multistep progression from initiation to malignancy - the role of chemicals and radiation
in multistep progression
CORE PAPER -II 08ZOOC13B
HUMAN GENETICS
UNIT-I
History of Human Chromosome Research - Denver Conference (1940) - Chicago
Conference (1966) - Paris Conference (1971) - Nomenclature of Human Chromosome.
UNIT-II
Identification of Human diploid chromosome - peripheral blood cultures - banding
techniques - G-band; Q-band; C-band; R-band - Identification of 23 pairs of Human
chromosomes by band position.
UNIT-III
Chromosomal syndromes: Autosomal syndromes - Sex chromosomal syndromes -
Structural chromosomal syndromes.
UNIT-IV
Prenatal diagnosis: Chorionic villi sampling - Foetoscopy, Ultrascopy - Amniocentesis.
b) Postnatal diagnosis: Peripheral blood leucocyte culture - Sister Chromatid Exchange -
Fragile site - Mitotic index. c) Genetic Counseling.
UNIT-V
Hereditary forms of Cancer - Oncogenes and Cancer - Chromosomes and Cancer - Cancer
and the environment.
CORE PAPER -III 08ZOOC13C
BIOINFORMATICS AND GENOMICS
Unit I
Biology of cells-Biology of Environment-Cell Energetics and Respiration- Calculus-
Integration.
Unit II
Internet and Intranet-Application of IT-Database Management Systems and
telecommunications.-Searches on MEDLINE, CD-ROM
Unit III
Chemical Bonding and Atomic and Molecular Orbital-X-ray crystallography of bio
molecules -Molecular mechanics and molecular dynamics of Protein, Pesticides,
Nucleotides.
Unit IV
Concept of molecular modeling-Genome data bank-microbial and cellular data banks
Analysis tools for sequence data banks.
Unit V
Introduction to Genomics- biological data modeling-Genomic mapping and single
nucleotide polymorphisms-Micro array gene expression analysis -Genomics databases
CORE PAPER -IV 08ZOOC13D
PRACTICAL - CELLULAR AND MOLECULAR BIOLOGY, HUMAN
GENETICS AND BIOINFORMATICS AND GENOMICS
CELLULAR AND MOLECULAR BIOLOGY:
1. Mendelian genetics calculations
2. Identification of different stages of meiosis and mitosis (slides).
3. Identification of sex chromatin in buccal smear test.
4. Preparation of mitosis in Onion root tip.
5. Squash preparation in Grasshopper testis.
HUMAN GENETICS:
1. Problems related to Mendelian laws.
2. Pedigree analysis.
3. Peripheral blood leukocyte culture for chromosomal studies.
4. Mitotic indices.
5. Sister chromatid exchange -determination
6. Micronucleus test.
7. Chromosomal disorders-Numerical, Structural.
BIOINFORMATICS AND GENOMICS
1. Bioinformatics and the Internet
2. Protein Sequence Analysis
3. Molecular Graphics and Evaluating Protein Structure
4. Small Molecule Generation and assessment
5. Free energy calculations.ELECTIVE PAPER -I 08ZOOC1EA
IMMUNOTECHNOLOGY
Unit-I
Introduction to Immune system; cellular and humoral immunity; complement; molecular
basis of Immune diversity. Host-parasite interaction; Immunity and infection: immunity
to bacteria, virus, protozoa, fungi and tumor.
Unit-II
Antigens and antigenicity. Polyclonal and monoclonal antibody production and
purification. Conjugation of antibody with enzymes, fluorochrome and toxin. Humoral
immune response and cell mediated immune response.
Unit-III
Immunity to infections. Immunological techniques: RIA, ELISA, Immunocytochemistry,
Immunofluorescence, Immunoblotting,
[
Unit-IV
Florescence antibody techniques, flow cytometry, Fluorescence in situ hybridization.
Immunopathology and immunological disorders.
Unit-V
Principles and methods of vaccine preparation - Edible vaccines. Antibody engineering
and structure. Cytokines and immunotherapy.