Proficiency courses in Enzyme Biotechnology(PART TIME)

Proficiency courses in Enzyme Biotechnology(PART TIME)

Course Structure
• Isolation of industrially important microorganisms from soil, water or air
• Screening for production of enzymes (amylase, protease, cellulase etc.)
• Strain improvement trials
• Mass production of the enzyme producing organisms
• Partial purification of the enzymes by any of the methods mentioned below
o Precipitation techniques
o Chromatography
• Characterization of the purified Enzyme
o SDS PAGE o Protein content o Enzyme assay
• Study of process efficiency
• Enzyme immobilization
• Assay of the enzyme after immobilization
• Documentation of experiments
• Report preparation