Post Graduate in Cellular Signalling

This paper gives the basic idea about functioning of cell and signelling
components, cell -cell interactions.
Objective: The main objective is to provide the detailed knowledge about cellular
signaling.
Goal: This course will provide the student with a comprehensive understanding of the
regulation of cellular function through signaling and will help them with a firm
foundation for a future career in basic research such as diseases like cancer which are
related to cellular signaling disfunctions.
UNIT I:
Signaling Components:Endocrine, Paracrine and Autocrine signaling; Signaling
molecules- Hormones (agonists and antagonists) NO; Receptor Classification: G proteins
linked Receptors, Enzyme activity associated receptors (TGF b,cytokine, Receptor
Tyrosine kinase ,Receptor guanylyl cyclase, Receptor Phospotyrosine phosphatase ,TCell
Receptor). Ion Channels as receptors, receptors involving in proteolysis (Wnt,
Hedgehog Hb, Notch/ Delta, NF -kb), intracellular receptor (NO. Pathway, Nuclear
receptor); Membrane bound and soluble receptors; membrane anchoring-myristoylation,
palmitoylation, Farnesylation, Geranylation, GPI anchor.
Unit II
Nuclear Receptors: Principle of signaling with nuclear receptors, classification and
structures; Transcriptional regulation by nuclear receptors-coactivators, co-repressors;
Regulation and variability of signaling; Signaling path ways via steroids hormones
receptors, Nuclease localized (retinoid, vit D3 and T3 hormones)
UNIT III
G protein Coupled signal transmission: GPCR Structure and classification ,ligand
binding domain; Signaling path ways via cAMP, ion Channel regulation, PJ3; Regulation
-GTPase super family and GTP hydrolysis; Phosducin and regulators of G protein
signaling (RGS).
Signaling pathway that control gene activity, TGFb Receptors ands Smad activation
;cytokine receptors ,and JAK -STAT path way; RTK and Ras activation; MAP Kinase
pathways; phosphoinositides as signal transducers; Signal induced protein cleavage (NFkB,
Notch /Delta, Wnt, Hedgehog)
UNIT IV
Integration of signals and gene controls: Responses of cells to environmental influences
control of cell fates by graded amounts of regulators, boundary creation by different
combination of transcription factors; Boundary creation by extra cellular signals;
Reciprocal induction and lateral inhibition; Integrating and controlling signals; Down
modulation of receptor signaling.
UNIT V
Regulation of cell cycle:Principle of cell cycle control, Structure and Function -CDKs,
Cyclin; regulation by proteolysis; G1/S phase Transition, G2/M transition;
Malfunction of signaling path ways: General aspects of tumor formation; Errors in cell
division; Oncogenes and tumor suppressor gene; Apoptosis.